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This paper introduces an algorithm to detect the least significant bit (LSB) steganography in audio files. Based on the sample pair analysis and the definition of complexity function in RS algorithm, the algorithm estimates the length of hidden messages using the statistical characteristic of LSB of audio samples and steganalysis audio files through the estimated length of hidden messages. This algorithm has low computing complexity and is easy to be implemented. Experimental results and theoretical verification show that this method is an effective method of audio LSB steganalysis.