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Mobile agent technology could be used to solve the problems on intrusion detection in network security area, while immune principle could supply reference to algorithm design of agent for detecting and analyzing data. In this paper, the detecting algorithm based on the immune principle, including the definition of problem domain, computation of affinity between the antibody and antigen,as well as between antibody, the generation of the detectors, has been elaborated. The algorithm of negative selection used in the agent has been improved. The experimental result indicates that the algorithm is more adaptive than the original one.