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In the agro-food industry there is a great need for rapid, sensitive and label-free biosensors for food quality control, for example the detection of pathogenic agents such as listeria or salmonella in dairy food samples. We are currently working on the development of a real-time monitoring system based on an interdigitated electrode DNA biochip for the detection of specific DNA target sequences. The biosensor contains four active areas on which DNA probes of various kinds can be immobilized, and is integrated into a microfluidic system. Impedimetric measurements of matching and non-matching synthetic target DNA sequences have been performed.