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The Cramer-Rao lower bound (CRLB) is derived for the channel estimation of direct sequence-code division multiple access ultra-wideband (DS-CDMA UWB) communication system. We have modified the channel model and reduced the number of estimation parameters. Furthermore, a low-complexity data-aided Least Square (LS) channel estimation algorithm is proposed for the modified model. In addition, the problem of designing orthogonal training sequence is discussed. Finally, numerical examples are provided to evaluate both the derived CRLB and the proposed channel estimation algorithm.