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In present, the medical infrared imaging technology is widely used in medical diagnosis and clinical therapy. People are paying much more attention to the development of this technology. However, it is hard to directly reflect the correlative information of the inner heat source. In order to make the diagnosis accurate, it is necessary to know the relationship between the surface temperature distribution and the inner heat source. In this paper, the surface temperature distribution of the tissue affected by the point heat source will be studied. In addition, the depth and the intensity of the point heat source will be deduced from the surface temperature distribution. By the simulation and the experiment, the results will be validated.