By Topic

Single-Cell Level Array of Yeast Cells in Pumpless Microfluidic Channels induced by Receding Meniscus

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)

We present a simple receding meniscus induced method to capture non-adherent yeast cells onto microwells inside microfluidic channels. Microwells were fabricated by capillary molding onto glass substrate using a UV curable polyurethane acrylate (PUA) solution, leading to well-defined, robust microstructures. A cell suspension of the budding yeast, Saccharomyces cerevisiae, was introduced into microfluidic channels by capillary filling and a receding meniscus was subsequently generated by evaporation. As the meniscus receded, yeast cells were spontaneously captured onto microwells at single-cell level. Using this cell-based platform, we observed the response of yeast cells upon stimulation by a mating pheromone (alpha-factor) by monitoring the expression of green fluorescent protein (GFP) with time. It was observed that alpha-factor triggered the expression of GFP at 60 min after stimulation and the fluorescence intensity was sustained for additional 60 min without changes.

Published in:

Sensors, 2006. 5th IEEE Conference on

Date of Conference:

22-25 Oct. 2006