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The fabrication of a blood extraction device integrated with an electrolyte-monitoring system is reported in this paper. The device has advantages in precise controlled dosage of blood extracted including the slightly damaged blood vessels and nervous system. Main components of the portable system are; the blood extraction device and electrolyte-monitoring system. The monitoring system consists of ISFET (ion selective field effect transistor) for measuring the concentration level of minerals in blood. In this work, the authors measured the level of 3 ions; Na+, K+ and Cl-. The 100 hollow-microneedles fabricated by synchrotron radiation deep X-ray lithography through PCT (plane-pattern to cross-section transfer) technique were revealed in 5times5 mm2 area. The total size of the blood extraction device is 2times2times2 cm3. The package is made from an acrylic socket consisting of microneedle array block, ion-sensors block, blood and reference reservoirs. ISFET was connected to an electrical circuit for monitoring the potential different.