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This study investigates the light propagation along the human meridian line and non-meridian line by diffuse reflectance measurement. Laser irradiates on the acupuncture points on the meridian line and the reference points on the nonmeridian line, and the diffuse light signals are detected on the meridian line and nonmeridian line respectively. Nineteen subjects are tested in this study. The medical statistical analysis software SPSS13.0 is used to analyze the results. Our study shows that the light propagates better along the meridian line than nonmeridian line (P<0.01).