By Topic

Rapid enumeration of bacterial cells in drinking water using a microfluidic device

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

6 Author(s)
Nobuyasu Yamaguchi ; Graduate Sch. of Pharm. Sci., Osaka Univ. ; Chieko Sakamoto ; Masumi Yamada ; Hiroyasu Nagase
more authors

The abundance of bacterial cells should be routinely monitored to maintain microbiological quality control of drinking water. We investigated the ability of a newly designed microfluidic device to determine bacterial numbers in freshwater at a density in the order of 104 cells/ml. A microfluidic device was designed and fabricated by polydimethylsiloxane (PDMS) and a thin glass slide (size: 48 mmtimes23 mm). Sheath fluid and fluorescently stained samples were placed in gastight syringes, and injected into the microfluidic device via Teflon tubes by syringe pumps at flow rates of 2.0mul/min. A fluorescent microscope-based system was used for enumeration of cells flowing rapidly through the microchannel. The numbers of fluorescent beads and E. coli cells at a density in the order of 10/ml determined by this on-chip flow cytometer were similar with the microscopic counts. The time required for quantitation by on-chip flow cytometer was only 10 min per sample, which was 3- to 5- fold less than microscopy, which usually requires concentration of bacterial cells onto a membrane filter. This simple enumeration method will contribute to the technical progress in microbiological quality control of drinking water

Published in:

2006 Bio Micro and Nanosystems Conference

Date of Conference:

15-18 Jan. 2006