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Electrocatalytic Determination of Glucose in a Ringer's Solution by Pulse-Voltammetry

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5 Author(s)
Lewandowksi, Jan J. ; Department of Artificial Organs, Cleveland Clinic Foundation ; Malchesky, Paul S. ; Nose, Yukihiko ; Moorman, Michael J.
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Improvement of the electrocatalytic method of glucose determination was achieved by applying the pulse voltammetry technique. This technique, compared. to previously applied cyclic voltammetry, enhances selectivity of a platinum-black working electrode for glucose measurement. A low catalytic load working electrode has been used which is less susceptible to poisoning and has longer term stability. With cyclic voltammetry previously applied, those electrodes were limited by their narrow concentration range for glucose detection. By applying a pulsing technique, these electrodes exhibit linearity up to a glucose concentration 200 mg/dl with correlation coefficients greater than 0.9. The pulse voltammetry diminishes interference by low molecular weight serum components such as serum urea. Concentrations from 20. to 40 mg/di had an interference of 4 percent compared to 40 percent with cyclic voltammetry. Further investigations are carried on to adopt pulse voltammetry for direct measurements of glucose in body fluids.

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Biomedical Engineering, IEEE Transactions on  (Volume:BME-33 ,  Issue: 2 )