By Topic

A Simple Technique to Measure the Rate and Magnitude of Shortening of Single Isolated Cardiac Myocytes

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Philips, Charkes M. ; Department of Physiology, Temple University School of Medicine ; Duthinh, Vuong ; Houser, Steven R.

A simple and inexpensive method for measuring the extent and rate of shortening of single myocytes was developed. The image of a single cardiac myocyte was focused through a 10x objective of an inverted microscope and projected onto a linear photodiode array having 256 self-scanning elements. This array was used as an edge detector to define cell length. The output from the array was fed into a digital-to-analog converter to produce an output voltage proportional to the myocyte length. In the present experiments the total scanning time for all the 256 elements was set at either 1.5 or 5 ms, thus allowing for detection of myocyte length changes during contraction. Results obtained using this system showed that the extent of shortening was 8.04 ±0.48 percent of the resting cell length (n = 15). The rate of shortening in these cells was 99.49 ±8.02 percent of the resting cell length/s. Similar results were obtained using a high speed film.

Published in:

Biomedical Engineering, IEEE Transactions on  (Volume:BME-33 ,  Issue: 10 )