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The conduction velocity of myoelectric potential along muscle fiber is known to be an index of the degree of muscular fatigue or muscular disease. When detecting the myoelectric potential by means of surface electrodes, the conduction velocity must be extracted from an apparently random wave of a myoelectric signal. In this paper, a method for determining conduction velocity is proposed based upon a zero-crossing time delay measurement with reference to the derivative of a myoelectric signal. The slope value of the input signal provides an effective criterion for rejecting undesired zero crossing caused by noise. This method needs no spectral analysis nor correlation calculation. Compared to another previously reported zero-cossing approach using digital filter preprocessing, it shows a more accurate and rapid estimation of velocity.