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We propose a novel collision resolution scheme termed the Optimal Protocol Tree (OPT), which is based on the tree search algorithm for RFID systems. The basic principle of OPT relies on taking advantage of the similarities in the identification strings of different tags, having the reader prompt the tags to send only the mutually exclusive sub-portion of their identification strings. The aim of OPT is to significantly reduce the total identification time, in order to render the deployment of dense RFID systems feasible. Simulation results are presented to demonstrate the performance of OPT, and the considerable improvement it provides with respect to the existing tree search protocols.