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Quantum Dot Probes for Monitoring Dynamic Cellular Response: Reporters of T Cell Activation

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4 Author(s)
Michael R. Warnement ; Dept. of Chem., Vanderbilt Univ., Nashville, TN ; Shannon L. Faley ; John P. Wikswo ; Sandra J. Rosenthal*

Antibody-conjugated quantum dots (QDs) have been used to map the expression dynamics of the cytokine receptor interleukin-2 receptor-alpha (IL-2Ralpha) following Jurkat T cell activation. Maximal receptor expression was observed 48 h after activation, followed by a sharp decrease consistent with IL-2R internalization subsequent to IL-2 engagement. Verification of T cell activation and specificity of QD labeling were demonstrated using fluorescence microscopy, ELISA, and FACS analyses. These antibody conjugates provide a versatile means to rapidly determine cell state and interrogate membrane associated proteins involved in cell signaling pathways. Ultimately, incorporation with a microfluidic platform capable of simultaneously monitoring several cell signaling pathways will aid in toxin detection and discrimination

Published in:

IEEE Transactions on NanoBioscience  (Volume:5 ,  Issue: 4 )