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The fluorescence pulse method as a tool to characterize the photosynthetic system of plants and algae

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1 Author(s)
B. Ruth ; Res. Centre for Environ. & Health, Inst. for Soil Ecology, Oberschleissheim, Germany

In order to obtain useful information in a reduced time spent, the method of chlorophyll fluorescence induction kinetics has been modified. An excitation pulse of rectangular shape with a power density P (0.1 mW/cm2-70 mW/cm2) and a pulse width T (T=0.55-2 ms) is used to induce the fluorescence of dark adapted plants. Its initial value Fo is the ground fluorescence. The further increase of the fluorescence intensity F(t) during the excitation pulse can be described by Fal·(1-exp(-t/Tal)). F o, Fal and Tal depend on e.g. the chlorophyll content, the herbicide concentration, water stress and P. Measurements show that a dark adaptation of 0.2 s is sufficient and that results can also be obtained even without any dark adaptation. The distance between measuring device and object can be varied between 0.5 m and 3 m

Published in:

Geoscience and Remote Sensing Symposium, 1994. IGARSS '94. Surface and Atmospheric Remote Sensing: Technologies, Data Analysis and Interpretation., International  (Volume:1 )

Date of Conference:

8-12 Aug 1994