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Large area real-time system for screening DNA libraries

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5 Author(s)
Gordon, J.S. ; Radiation Monitoring Devices Inc., Watertown, MA, USA ; McGann, W. ; Daley, K. ; McConchie, L.
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A nuclear imaging system based on a position-sensitive photomultiplier tube (PSPMT) and scintillating fiber optics is described. A real-time, large-area imaging system has been constructed and tested using realistic phantoms and biological samples. This prototype, which has an active area of 112 cm2 was found to be more than 100 times more sensitive for detection of 32P radiation than film and provided a spatial resolution of approximately 1.7 mm. By choosing suitable fiber dimensions and packing methods on the PSPMT this system is capable of producing images over an area equal to X-ray film and with spatial resolution approaching 0.5 mm. The digital nature of the image allows a great deal of freedom in processing and displaying data, including automatic identification and location of the desired features

Published in:

Nuclear Science, IEEE Transactions on  (Volume:39 ,  Issue: 5 )

Date of Publication:

Oct 1992

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