Cart (Loading....) | Create Account
Close category search window
 

Analyses of Functions and Interactions of Protein Molecules by Single Fluorescent Molecular Imaging

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

The purchase and pricing options are temporarily unavailable. Please try again later.
1 Author(s)
Funatsu, T. ; Lab. of Bio-Anal. Chem., Tokyo Univ.

Single-molecule imaging is a useful technique for analyzing functions of proteins and protein-protein interactions. As an application of this technique, we investigated the mechanism of protein folding assisted by chaperonin GroEL. We showed that the chaperonin reaction cycle consisted of two successive timers of ~3 s and ~5 s duration. It suggested a new intermediate state that ensured efficient encapsulation of substrate protein. As another approach to study protein-protein interactions, we are now developing a microdevice that enables to sort supra-macromolecules. The solution containing organelles, of which component is fluorescent, and thermosensitive polymer was introduced to a Y-shaped microchannel. The sol-gel transition was locally induced by site directed infrared laser irradiation, resulting in plugging the microchannel and sort out the biomolecules

Published in:

Optical MEMS and Their Applications Conference, 2006. IEEE/LEOS International Conference on

Date of Conference:

21-24 Aug. 2006

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.