Cart (Loading....) | Create Account
Close category search window
 

Design of RT-PCR Primers Based on Informational Method

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Makino, K. ; Showa Hosp., Akane Med. Corp. ; Hirano, H.

To detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because the most of the traditional computer protocol succeeded less than 20%. The 90% of our primers functioned after following checking 1) GC Contents Check; 2) Homologous Check: [versus a: GAPDH, b: Profilin, c: beta-actin]; 3) Self-Homology Check). We also added 4: 5'-, & -3' homology checking, and 5: gene family checking to target into a real mRNA out of similar sequences. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from gene family in which there are many similar sequences

Published in:

Innovative Computing, Information and Control, 2006. ICICIC '06. First International Conference on  (Volume:3 )

Date of Conference:

Aug. 30 2006-Sept. 1 2006

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.