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The impedance characteristics of gold-plated indium-tin-oxide microelectrodes immersed in culture medium (MEM) are described and compared with the impedance characteristics observed when those microelectrodes are immersed in isotonic saline. For microelectrode areas of approximately 100 mu m 2, applied voltage levels of 5, 50 and 100 mV, and for frequencies of from 100 Hz to 10 kHz the resistance, capacitance, capacitive reactance, and total impedance are given as a function of frequency both in culture medium and in saline. The results, which hold for current densities ranging from 0.45 to 700 pA/ mu 2, are compared. Also given are the alpha and K values determining the frequency characteristics of the interface resistance and capacitance in medium and in saline.