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Identification of pathogenic fungi using computational and molecular biological approaches

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4 Author(s)
Chandio, S.H. ; Dept. of Comput. Sci., Nebraska Univ., Omaha, NE ; Bastola, D.R. ; Iwen, P.C. ; Hinrichs, S.H.

The lack of rapid diagnostic procedures is a major obstacle in the successful management of fungal disease. Most of the methods now used in the microbiology laboratory include growth-based phenotypic testing. However, polymerase chain reaction (PCR) has allowed for rapid identification of organism without the need for a growing culture. An ion-paired reverse phase chromatography (IP-RP)-based assay using high performance liquid chromatography (HPLC) was developed with commercially available IP-RP-HPLC system called the WAVE to differentiate medically important fungi. Universal fungus-specific primers and restriction fragment length polymorphism (RFLP) of PCR amplicon were done prior to WAVE analysis. The assay was enhanced when combined with a searchable relational database of retention time. Discrimination among closely related fungal species was possible by evaluation of distinct-retention time patterns. This assay is simple, rapid, and allows for the identification of medically important fungi by searching the database with the information obtained from PCR-WAVE analysis

Published in:

Electro Information Technology, 2005 IEEE International Conference on

Date of Conference:

22-25 May 2005

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