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In contrast to the commonly employed single-ended delay lines, the employment of differential signaling may alleviate the occurrence of crosstalk and improve the signal integrity. This paper qualitatively investigates the time-domain reflection (TDR) and time-domain transmission (TDT) waveforms for the single-ended and differential delay lines with the serpentine and flat spiral routing schemes. A numerical formula is then proposed to quantitatively predict the voltage levels of the saturated near-end and far-end propagating crosstalk noises among the sections of differential delay lines. Signal waveforms and eye diagrams of the four basic routing schemes are obtained by HSPICE simulations, demonstrating that the combination of differential signaling and flat spiral layouts can exhibit the best delay-line performance. Furthermore, both the TDR and TDT measurements for differential delay lines are performed to validate the exactitude of proposed analyses.