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Investigation of temperature gradient effects on neurite outgrowth in nerve cells using a microfabricated heater structure

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4 Author(s)
Jain, A. ; Dept. of Mech. Eng., Stanford Univ., CA, USA ; Ness, K.D. ; Fishman, H.A. ; Goodson, K.E.

A novel heater structure capable of producing precisely controlled temperature gradients at cellular length scales is microfabricated. Protocols for successful culturing of RGC and PC12 cells on the heater surface are developed, and the heater structure is used to investigate the effect of spatial temperature gradients on neurite outgrowth in these cells. The results are discussed in the light of a recently developed theory for chemically-mediated thermotaxis.

Published in:
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on

Date of Conference: 12-15 May 2005

Page(s):
398 - 399
Print ISBN:
0-7803-8711-2
INSPEC Accession Number:
8671433
Digital Object Identifier :
10.1109/MMB.2005.1548487

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