By Topic

A single-cell electroporation device with integrated perfusion channels for efficient intracellular delivery

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Khine, Michelle ; Dept. of Bioeng., California Univ., Berkeley, CA, USA ; Lau, A. ; Seo, Jeonggi ; Lee, Luke P.

We present here a single-cell electroporation device with integrated perfusion channels to efficiently deliver impermeable substances into a cell. Each sequestered cell is subjected to an electric field to induce localized transient non-selective pores in a portion of the cell membrane. After pores are locally induced on a portion of the cell membrane, individually addressable capillary channels release pre-loaded compounds of interest into the cell. This approach conserves reagents; moreover cells remain separated from reagents until time of introduction. To demonstrate the effectiveness of our device design, we electroporate Hela (human cervical cancer) cells using low applied voltages (<1 V) and characterize permeation by measuring time dependent current traces. Subsequently, we insert cell impermeable propidium iodide and characterize its deliver/ via fluorescent intensity measurements.

Published in:

Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on

Date of Conference:

12-15 May 2005