Skip to Main Content
We proposed a mathematical model for the micro-dynamics of cultured cells measured with ECIS (Electrical Cell-substrate Impedance Sensing system. Our mathematical model is composed of culture medium impedance between cells (Zsol), cell impedance (Zc), and polarization impedance of the electrode (Zp). It was found that whether cell-to-cell or cell-to-substrate gaps caused the changes in the impedance could be determined based on the changes in the total resistance and reactance (capacitance). Cell-to-cell gaps mainly affects the total resistance value. Cell-to-substrate gaps mainly affects total capacitance value. Based on these results, when the cultured endothelial cells (HUVEC) were stimulated with estrogen for 40 hours, it was proved that the cell-to-cell distances decreased, even though the cell-to-substrate distances slightly increased. This result suggests that the barrier function of endothelium is fortified by estrogen.
Date of Conference: 12-15 May 2005