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In this paper, the frequency-dependent characteristic impedance and propagation constant of lossy transmission lines have been extracted from one-port time-domain reflectometry (TDR) measurements. Nonphysical resistance (R), inductance (L), conductance (G) and capacitance (C) (RLGC) models have been developed for simulating lossy transmission lines using the extracted data. The extraction method has been demonstrated for transmission lines on an organic substrate such as coplanar lines. Using the extracted data and nonphysical RLGC models, the simulation results show good correlation with TDR measurements for coplanar lines.