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The objective of this research is to evaluate e-learning Websites for university in Thailand by using quality criteria which based on IEEE 1061 and ISO/IEC 9126. In this research, we determine 6 quality aspects (include 2 new aspects) such as functionality, reliability, usability, efficiency, maintainability and portability. In the research process, we categorize subjects into 5 groups. The result has found that the average of quality in e-learning Websites is 50.34% and this can be exploited as beneficial information for e-learning Webmaster to evaluate and improve the quality of e-learning Website according to the proposed quality model for optimizing the effective e-learning.