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Electrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels

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5 Author(s)
Nishizawa, M. ; Dept. of Bioeng. & Robotics, Tohoku Univ., Sendai, Japan ; Kaji, H. ; Tsukidate, K. ; Hashimoto, M.
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The use of microfluidic systems is a recent trend in diagnostic assays and bioreactors based on protein and cellular engineering. Most microfluidic biodevices would require combination with a technique to pattern proteins and living cells within microchannels just prior to the use of the devices, since these bio-elements are delicate against desiccation, oxidation and heat. Here, we present a simple, electrochemical method to locally immobilize proteins and cells on substrate surface, easily applicable to the microfluidic system. The integration of the technique into a microfluidic device and the on-demand immobilization of proteins and cells were achieved. The sandwich immunoassay within the microchannel was demonstrated, in which a series of protocols from immobilization to measurement can be carried out under physiochemical conditions.

Published in:

Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on  (Volume:1 )

Date of Conference:

5-9 June 2005