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Video segmentation has been an important and challenging issue for many video applications. Usually there are two different video segmentation approaches, i.e., shot-based segmentation that uses a set of key-frames to represent a video shot and object-based segmentation that partitions a video shot into objects and background. Representing a video shot at different semantic levels, two segmentation processes are usually implemented separately or independently for video analysis. In this paper, we propose a new approach to combine two video segmentation techniques together. Specifically, a combined key-frame extraction and object-based segmentation method is developed based state-of-the-art video segmentation algorithms and statistical clustering approaches. On the one hand, shot-based segmentation can dramatically facilitate and enhance object-based segmentation by using key-frame extraction to select a few key-frames for statistical model training. On the other hand, object-based segmentation can be used to improve shot-based segmentation results by using model-based key-frame refinement. The proposed approach is able to integrate advantages of these two segmentation methods and provide a new combined shot-based and object-based framework for a variety of advanced video analysis tasks. Experimental results validate effectiveness and flexibility of the proposed video segmentation algorithm.