By Topic

Multithermal DNA micro-array chip for rapid DNA melting temperature measurement and advanced SNP discrimination

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
S. Petronis ; Dept. of Micro & Nanotechnol., Tech. Univ. of Denmark, Denmark ; D. T. Ganzhorn ; C. B. V. Christensen ; M. Dufva

This paper describes a micromachined device dedicated to measure DNA target-probe melting temperatures and to discriminate multiplex single nucleotide polymorphisms on a standard DNA micro-array slide. The microfluidic system of the chip divided the micro-array slide into eight washing chambers, each providing different thermal conditions for stringent washing. Melting temperature of DNA target-probe hybrids measured by the chip corresponded well to the melting curves determined by standard methods. The long DNA capture probes provided higher melting temperatures then shorter probes, and the perfectly matched hybrids had higher melting temperatures than the corresponding hybrids containing SNP mutation, as expected. Due to the wide washing temperature range provided simultaneously by the chip, it was possible both to create and to find optimal washing temperatures for discrimination of several SNPs in a single experiment.

Published in:

18th IEEE International Conference on Micro Electro Mechanical Systems, 2005. MEMS 2005.

Date of Conference:

30 Jan.-3 Feb. 2005