Cart (Loading....) | Create Account
Close category search window
 

Polymer microfluidics coupled with MALDI mass spectrometry

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Wang, Y.-X. ; Maryland Univ., College Park, MD, USA ; Li, Y. ; Zhou, Y. ; Lee, C.S.
more authors

The offline coupling of polymer microfluidic chips to mass spectrometry (MS) through electrospray deposition of analyte onto matrix-assisted laser desorption/ionization (MALDI) targets for MALDI-MS analysis is presented. Using polycarbonate (PC) microfluidic chips with integrated hydrophobic membrane electrospray tips, selected peptides and proteins were eluted onto a stainless steel MALDI target via electrospray deposition, followed by off-line MALDI-MS analysis. A variety of matrix compositions were evaluated, and the resulting film morphology was studied via scanning electron microscopy. Microchip electrospray deposition is found to yield excellent homogeneity of peptide crystals, and exhibits significantly improved reproducibility during MALDI-MS analysis. Using this approach, a detection limit of 3.5 pmol is demonstrated for angiotensin.

Published in:

Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on

Date of Conference:

30 Jan.-3 Feb. 2005

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.