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In this paper we present a novel sequence-based RF localization algorithm called Ecolocation. Our algorithm determines the location of unknown nodes by examining the ordered sequence of received signal strength (RSS) measurements taken at multiple reference nodes. We employ a constraint-based approach that provides for robust location decoding even in the presence of random RSS fluctuations due to multi-path fading and shadowing. Through extensive systematic simulations, and a representative set of real mote experiments, we show that over a wide range of settings Ecolocation performs better than other state of the art approaches in terms of localization accuracy and precision.