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In the present study, totally 40 rats Wistar Albino weighing 271±12 gr were used. They were divided into four groups; control (n=10), control group exposed to AC magnetic field (n=10), diabetic groups (n=10) and diabetic group exposed to AC magnetic field (n=10). 20 rats were in the experimental group exposed to AC magnetic field, 10 of them (control) having magnetic field and the other ten were diabetic group. The rats in the experimental groups were exposed to a smooth magnetic field with 5 mT force in 50 Hz frequency during four weeks 3 hours a day, and diaphragm and muscle strips (0.053±0.006 g.) were taken from the rats in the each group. Muscle twitch measured by isometric transducers was observed through digital storage oscilloscope, and put into computer in order for analysis. Of the isometric twitch tensions (Ps), contraction time (CT), one-half relaxation time ( 1/2 RT) were determined. In conclusion, the data analysed revealed that there was a significant difference between the isometric twitch parameters CT, 1/2 RT and isometric contraction force Ps taken from muscle strips belonging to the four groups and there were significant difference between the other parameters measured.