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We characterize a droplet-based biochemical reaction system with the features of droplet self-positioning, self-mixing and self-alignment optics on a micro-patterning chip. The phenomenon of droplet self-positioning is explained as follows: the different texture zones generate a gradient of surface tension force to manipulate the droplet. Droplet-based reagents can be transported, precisely positioned, and mixed on the detection zone without any power source. This system demonstrates droplet manipulation, and biochemical reaction on the micro-patterning chip with a self-alignment micro lens.