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In order to prevent a loss of spectral efficiency due to the use of a cyclic prefix (CP), a CP reconstruction (CPR) method, such as residual intersymbol interference cancellation (RISIC), has recently been developed. In this letter, we apply the RISIC method to an Alamouti space-time block coded (STBC) orthogonal frequency division multiplexing (OFDM) system with insufficient CP. It is shown that in the STBC OFDM, tail cancellation as well as cyclic restoration of the RISIC should be repeated. An iterative channel estimation method for the STBC OFDM system with CPR is proposed. The performance of the proposed method is evaluated by computer simulation in a multipath fading environment.