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In this paper, we present a new microwave-based method to make images of fiber structure, e.g., to locate knots in wood. Evaluation of Maxwell's equations for an electromagnetic wave propagating in an anisotropic media (wood) shows that the polarization of the wave depends on the local fiber orientation in the sample. Experiments support the theoretical evaluation showing that knots can be followed in images of polarimetric parameters, reconstructed from multiple monostatic measurements. The equipment works at low intensity and is, therefore, virtually harmless to human beings; furthermore, it can be made portable and be operated by a single person.