By Topic

Building phylogenetic trees from binary oligonucleotide fingerprint vectors

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
A. Figueroa ; Dept. of Comput. Sci., California Univ., Riverside, CA, USA ; Z. Liu ; R. Mancini-Jones ; J. Borneman
more authors

Oligonucleotide fingerprinting of ribosomal RNA genes (OFRG) is a method that permits the identification of microorganisms through ribosomal RNA gene (rDNA) analysis. OFRG sorts arrayed rDNA gene clones into clusters through a series of hybridization experiments, each using a single oligonucleotide probe. This series of hybridization experiments generates a "fingerprint" for each rDNA done. The fingerprints are binary vectors that specify whether the probes hybridized or did not hybridize to the clones. Identification of the microorganisms is accomplished by clustering fingerprints from unidentified clones with those from identified clones. The most accurate taxonomic classifications from rDNA analysis are often obtained through complete nucleotide sequence analysis. However, the direct information that we acquire from OFRG is the presence or absence of a specific substring of nucleotides in the unidentified rDNA sequence. This paper provides several ways to associate information to the set of fingerprints obtained by OFRG.

Published in:

Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE  (Volume:2 )

Date of Conference:

1-5 Sept. 2004