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Cell-based biosensors incorporate cells as sensing elements that convert a change in immediate environment to signals conducive for processing. It characterizes with high sensitivity, excellent selectivity and fast response. Cell-based biosensors have been implemented for a number of applications ranging from pharmaceutical screening to environmental pollutant detection. This work demonstrate a novel cell-based biosensor following the principle of light-addressable potentiometric sensor and field effect transistor for detecting the action potential of single living cell under drugs. The experimental results agree with our expectation.