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Developing metrology for tissue engineering: collinear optical coherence and confocal fluorescence microscopies

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5 Author(s)
Landis, F.A. ; Polymers Div., Nat. Inst. of Stand. & Technol., Gaithersburg, MD, USA ; Cicerone, Marcus T. ; Cooper, J.A. ; Washburn, Newell R.
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We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on tissue engineered medical products (TEMPs) in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by comparing the images of fluorescently stained osteoblasts cultured in a polymeric TEMP for 21 days and 10 weeks.

Published in:

Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on

Date of Conference:

15-18 April 2004