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In vivo multi lifetime discrimination using time domain fluorescence imaging

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2 Author(s)
Lesage, F. ; ART Recherches et Technol. Avancees Inc., Saint-Laurent, Que., Canada ; Belenkov, A.

Time domain imaging is presented as a method to perform in vivo fluorophore concentration reconstructions in the presence of varying lifetimes. In particular, using the SAMI™platform, target and endogenous fluorescence are shown to be distinguishable. Reconstruction of a solid Cy5.5 subcutaneous inclusion in a mouse is done with background endogenous fluorescence originating from the food in the stomach. The discrimination between both sources of fluorescence is done by a simple method thereby providing a better reconstruction.

Published in:

Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on

Date of Conference:

15-18 April 2004

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