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Remote detection of ciliary movement by fiber optic laser-Doppler spectroscopy

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2 Author(s)
Verdugo, P. ; Center of Bioeng., Washington Univ., Seattle, WA, USA ; Goldborne, C.E.

The development and validation of a fiber-optic laser-Doppler spectrometer to measure the movement of cilia in situ is described. The spectrometer was calibrated with reference to high-speed cinematography. The transfer function demonstrates an excellent correlation between these two methods in a range of frequency of ciliary beat extending from 3 to 38 Hz. Results are reported showing that this instrument can reliably and reproducibly detect and record ciliary activity inside the airways and the oviduct. The fiber-optic probe of the laser spectroscope can be easily introduced via the biopsy channel of a bronchoscope or laparoscope.

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Biomedical Engineering, IEEE Transactions on  (Volume:35 ,  Issue: 5 )