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Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions

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4 Author(s)
Prabhat, P. ; Dept. of Electr. Eng., Univ. of Texas, Richardson, TX, USA ; Ram, S. ; Ward, E.S. ; Ober, R.J.

The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.

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NanoBioscience, IEEE Transactions on  (Volume:3 ,  Issue: 4 )