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We have searched for antistatic agent behavior by surface resistivity and space charge measurement. In a structure of three layer (PET/PE/EVA) that EVA layer is mixed with an antistatic agent, we measured the humidity characteristic of the surface resistivity (ρs) in order to observe the influence of a PE layer against the behavior of antistatic agent. As a result, we confirmed that EVA surface resistivity were influenced by character of PE layer. Next, we searched for the diffusion speed of the antistatic agent by measuring the change in the surface resistivity (ρs) of the PE layer when we laminated two films of EVA film and PE film together at 403 K (130 °C). As a result, we confirmed that diffusion speed of antistatic agent varied with PE layer. Finally we tried to observe diffusion of antistatic agent from EVA to PE using pulse electroacoustic method. We could observe the mobility of a space charge in PE layer.