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An acceleration-based wireless motion sensing system (WMOSS) was developed for sports science applications. It is able to provide real-time information on human motions other than the conventional vision-based motion sensing systems. In this paper, we will present the working principles and system integration issues of the device. The initial calibration, signal processing and 3D self-calibration functions for the device algorithm were developed to optimize the system performance. Experiments were also conducted to analyze human motions using the WMOSS. The results from these experiments are also discussed in this paper.