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A coaxial driven planar slot antenna resonant in the microwave regime was used to detect changes in protein conformation induced by temperature modulation. Simultaneous dielectric and UV/VIS spectroscopy measurements were obtained by attaching the slot antenna to a fused-quartz UV/VIS sample holder. Similar thermodynamic parameters (midpoint temperature and enthalpy) were obtained from both techniques over a wide concentration range and under a variety of pH condition. Here we demonstrate that a near-field antenna can be used to detect changes in conformation at very low protein concentrations, and that the protein is not destabilized by the presence of microwave power.
Microwave Symposium Digest, 2004 IEEE MTT-S International (Volume:3 )
Date of Conference: 6-11 June 2004