By Topic

Analysis of electrotonic coupling in patterned neuronal networks

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $31
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

7 Author(s)
Lauer, L. ; Max Planck Inst. for Polymer Res., Mainz, Germany ; Vogt, A.K. ; Kauff, C. ; Nelles, G.
more authors

Microcontact printing of laminin is known as an efficient approach for guiding neuronal cell migration and neurite outgrowth on artificial surfaces. In the present study, ultrathin (∼250 μm) brain stem slices of Sprague-Dawley rats (E15-E18) were cultured on laminin-patterned substrates such that neuronal cells migrating out of the slices formed grid-shaped neuronal networks along the geometry defined by the pattern. The interconnections between neighbouring pairs of neurons within these artificial networks were assessed electrophysiologically by double patch-clamp recordings and optically by microinjection of fluorescent dyes. Both functional and electrotonic synapses were detected. Based on the recorded data and simulations in PSpice, an electrical model for electrotonically coupled cells was derived. In this model the neuritic pathway is described as a cylindric cable and gap junctions are represented by an ohmic resistor. Applying this model in the data analysis the average inner radius of neurites could be determined to be ∼0.1 μm. In addition, evidence was found for a correlation between the pathwidth of the applied pattern and the diameter of neurites growing along these paths.

Published in:

Nanobiotechnology, IEE Proceedings -  (Volume:151 ,  Issue: 3 )