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For a multichannel quartz crystal microbalance (MQCM) device, the key problem in design is to minimize the frequency interference between adjacent channels. In this paper, the effects of interchannel separation on the resonance frequencies and frequency interference of the device are studied. The results show that, for the channels with the same size, the resonant modes will appear alternatively, and the frequency interference keeps oscillating in a very small scope in the cases of large interchannel separations. Based on the studies, the considerations for MQCM design also are discussed.