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An FIR filter as pre-emphasis has been used to counteract intersymbol interference (ISI) in high-speed backplane data transmission. A MATLAB program has been developed for optimisation of the FIR filter under LMS criterion. Both a symbol-spaced FIR (SSF) filter and fractionally spaced FIR (FSF) filter are comparatively studied. The determination of tap number and preference of FSF to SSF are discussed.