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Parallel fluorescence detection of single biomolecules in microarrays using a diffractive optical designed 2×2 fan-out element

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7 Author(s)
Blom, Hans ; Dept. of Microelectron. & Inf. Technol., R. Inst. of Technol., Kista, Sweden ; Johansson, M. ; Hedman, A.-S. ; Lundberg, L.
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We have developed a multifocal diffractive optical fluorescence correlation spectroscopy system for parallel excitation and detection of single tetramethylrhodamine (TMR-4-dUTP) biomolecules in microarrays (Gene chips). Multifocal excitation was made possible through the use of a 2 × 2 fan-out diffractive optical element (DOE or kinoform) with uniform intensity in all four foci. Results of parallel excitation and detection in a high-density microarray of circular wells (o 70 μm), using the multifocal diffractive optical element designed 2 × 2 fan-out, show single biomolecule (TMR-4-dUTP) sensitivity in all foci, simultaneously. Future drug screening or functional genome analysis can hereby profit from this high sensitivity, parallel, fluorescence detection principle in biochip microarrays.

Published in:

Lasers and Electro-Optics Europe, 2003. CLEO/Europe. 2003 Conference on

Date of Conference:

22-27 June 2003