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A threshold detector for single-trial P300 detection has been evaluated. The detector operates on the 0-4 Hz band, isolated from the raw electroencephalogram using low-pass filtering, wavelet transforms, or the piecewise prony method (PPM). A detection rate around 70% was found, irregardless of stimulus type, interstimulus interval (ISI), probability of occurrence (Pr) of the target stimuli, intrasession and intrasession effects, or filtering method. This suggests that P300-based brain-machine interfaces can use an ISI as short as 1 s and a Pr of 45%, to increase throughput.