We are currently experiencing intermittent issues impacting performance. We apologize for the inconvenience.
By Topic

In vitro investigation of the procoagulant of silver ion

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Bhatt, B.A. ; New Jersey Inst. of Technol., Newark, NJ, USA ; Kristol, D. ; Spillert, C.R.

Silver ion, a potent antimicrobial and procoagulant, is hypothesized to achieve its haemostatic effect by binding anticoagulant proteins. We performed in vitro studies to gain more insight into its mechanism of action. 10 μL of 5% AgNO3 was added to 1 mL human plasma (n = 4), forming a precipitate. The precipitate was recovered through centrifugation and iteratively washed with saline. The supernatant(s) and precipitate were mixed with Coomassie blue stain and protein content was measured via spectrophotometric absorbance. An analysis of variance indicated significant (p<0.0001) differences between all groups. Multiple comparison tests showed a significant (p<0.001) increase in absorbance from the final wash and dye blank to the precipitate, indicating the presence of proteins in the precipitate. Silver ion may be precipitating anticoagulant proteins as its mechanism of action.

Published in:

Bioengineering Conference, 2004. Proceedings of the IEEE 30th Annual Northeast

Date of Conference:

17-18 April 2004